Hotspot

rps16-trnQ-UUG

LSC · 5875-7267 · 1393 bp

Back to Monanthes

Pi 0.0121
MarkerSeek score 57.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1393
variable_sites
32
pi
0.0121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1464-1484 bp 1.0 81.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA ATAAATAGGTGTGGGGCGTG 45.0 50.0 58.1 57.7 1523-1543 bp 1.0 78.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1494-1514 bp 1.0 76.1
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1456-1476 bp 1.0 75.8
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1463-1483 bp 1.0 75.2