Hotspot

ycf1

SSC · 128312-132298 · 3987 bp

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Pi 0.0282

MarkerSeek score 58.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3987
variable_sites: 167
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGTTTTCGGGGAGAACC	ATGCGTGCTCAAAGACGTAA	50.0	45.0	58.0	58.2	674-692 bp	1.0	85.2
ycf1_p2	AGTTGTTTTCGGGGAGAACC	GTTCACGAAAAGCCAAACGT	50.0	45.0	58.0	57.8	838-862 bp	1.0	84.8
ycf1_p3	TTTCGGGGAGAACCAAAAGG	ATGCGTGCTCAAAGACGTAA	50.0	45.0	58.0	58.2	668-686 bp	0.666667	69.6
ycf1_p4	TTTCGGGGAGAACCAAAAGG	GTTCACGAAAAGCCAAACGT	50.0	45.0	58.0	57.8	832-850 bp	0.666667	69.2
ycf1_p5	AGCTTCCATTCCCCAAACTG	GTTCACGAAAAGCCAAACGT	50.0	45.0	58.1	57.8	806-824 bp	0.666667	68.8