Hotspot

ndhE-ndhG

SSC · 122428-122651 · 224 bp

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Pi 0.0357

MarkerSeek score 78.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 224
variable_sites: 12
pi: 0.0357
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGATCAATCC	TTTTAGCTGCTTTGATCGGC	50.0	45.0	58.3	57.1	337-342 bp	1.0	74.1
ndhE-ndhG_p2	CGGCTCGTGATCAATCCATA	TTTTAGCTGCTTTGATCGGC	50.0	45.0	57.6	57.1	334-339 bp	1.0	73.4
ndhE-ndhG_p3	AGCTCAAGACACATAAGGGC	TTTTAGCTGCTTTGATCGGC	50.0	45.0	57.6	57.1	367-372 bp	1.0	73.3
ndhE-ndhG_p4	GCTCAAGACACATAAGGGCT	TTTTAGCTGCTTTGATCGGC	50.0	45.0	57.6	57.1	366-371 bp	1.0	73.3
ndhE-ndhG_p5	TTTCGGCTCGTGATCAATCC	CTTTTAGCTGCTTTGATCGGC	50.0	47.6	58.3	58.2	338-343 bp	1.0	71.8