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clpP-psbB

LSC · 75548-76018 · 471 bp

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Pi 0.0226

MarkerSeek score 76.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 16
pi: 0.0226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CACGATGCATCTTCCTCTCC	GAACAGAAAGCAAACGACCG	55.0	50.0	58.1	58.0	581-597 bp	1.0	84.0
clpP-psbB_p2	CCACGATGCATCTTCCTCTC	GAACAGAAAGCAAACGACCG	55.0	50.0	58.1	58.0	582-598 bp	1.0	84.0
clpP-psbB_p3	CGATGCATCTTCCTCTCCAG	GAACAGAAAGCAAACGACCG	55.0	50.0	57.8	58.0	579-595 bp	1.0	83.9
clpP-psbB_p4	TATACGTCAACCCACGATGC	GAACAGAAAGCAAACGACCG	50.0	50.0	57.8	58.0	593-609 bp	1.0	83.3
clpP-psbB_p5	CACGATGCATCTTCCTCTCC	GTATAGGGCCATTGAACCGG	55.0	55.0	58.1	58.1	634-650 bp	1.0	82.9