Hotspot

rpl33-rps18

LSC · 71315-71484 · 170 bp

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Pi 0.0516

MarkerSeek score 72.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 13
pi: 0.0516
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CTCAGTCAAACTCGGACTCG	CAAGGGGCAGTTGCTTCTTA	55.0	50.0	58.0	58.1	1337-1343 bp	1.0	81.0
rpl33-rps18_p2	GGTTCATGGCCAAGGGTAAA	CAAGGGGCAGTTGCTTCTTA	50.0	50.0	58.1	58.1	1056-1057 bp	1.0	80.8
rpl33-rps18_p3	TGGGAAGGGGTGAAGAAGAT	CAAGGGGCAGTTGCTTCTTA	50.0	50.0	57.9	58.1	1464-1470 bp	1.0	80.7
rpl33-rps18_p4	CTCAGTCAAACTCGGACTCG	ATACAAGGGGCAGTTGCTTC	55.0	50.0	58.0	58.2	1340-1346 bp	1.0	80.4
rpl33-rps18_p5	GTGACTAATCCCCTTCCCCT	CAAGGGGCAGTTGCTTCTTA	55.0	50.0	58.1	58.1	1423-1429 bp	1.0	80.3