Hotspot

trnM-CAU-atpE

LSC · 55640-55803 · 164 bp

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Pi 0.0695

MarkerSeek score 75.10

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 13
pi: 0.0695
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TGGACCGAACCTTTTGTCAG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.0	58.0	620-629 bp	1.0	85.1
trnM-CAU-atpE_p2	GCTCCATGTGCTCTGATTCA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.0	58.0	953-954 bp	1.0	84.1
trnM-CAU-atpE_p3	GCTCCATGTGCTCTGATTCA	GGAAATACGTTGGCCTAGCA	50.0	50.0	58.0	58.0	1468-1469 bp	1.0	82.6
trnM-CAU-atpE_p4	GCTCCATGTGCTCTGATTCA	GCACGAGCACGAAAAATTGA	50.0	45.0	58.0	58.0	1541-1542 bp	1.0	82.5
trnM-CAU-atpE_p5	TGGACCGAACCTTTTGTCAG	GCACGAGCACGAAAAATTGA	50.0	45.0	58.0	58.0	1208-1217 bp	1.0	82.5