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rps4-trnT-UGU

LSC · 49127-49472 · 346 bp

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Pi 0.0347

MarkerSeek score 73.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 18
pi: 0.0347
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTCCGGTTTTGGGCCTTTTA	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.9	57.9	491-497 bp	1.0	83.6
rps4-trnT-UGU_p2	TTCCGGTTTTGGGCCTTTTA	ATGGTCATCGGTTCGATTCC	45.0	50.0	57.9	57.8	459-465 bp	1.0	83.1
rps4-trnT-UGU_p3	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	430-436 bp	1.0	82.8
rps4-trnT-UGU_p4	TTCCGGTTTTGGGCCTTTTA	CTGTTATGTGAGCCCGCTTA	45.0	50.0	57.9	57.7	512-518 bp	1.0	82.0
rps4-trnT-UGU_p5	TTCCGGTTTTGGGCCTTTTA	TGTTATGTGAGCCCGCTTAG	45.0	50.0	57.9	57.7	511-517 bp	1.0	82.0