Hotspot

psbC-trnS-UGA

LSC · 38012-38246 · 235 bp

Back to Momordica

Pi 0.0369

MarkerSeek score 72.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 13
pi: 0.0369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTTATGGCATGCAGGAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.2	58.1	412-416 bp	1.0	80.3
psbC-trnS-UGA_p2	TTTATGGCATGCAGGAAGGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.2	58.1	423-427 bp	1.0	79.7
psbC-trnS-UGA_p3	TTTATGGCATGCAGGAAGGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.2	58.2	358-362 bp	1.0	79.4
psbC-trnS-UGA_p4	TTTATGGCATGCAGGAAGGG	TGGTTGATAGCTCCGGTCTT	50.0	50.0	58.2	58.4	413-417 bp	1.0	77.3
psbC-trnS-UGA_p5	TTTATGGCATGCAGGAAGGG	TAGCTCCGGTCTTGAAAACC	50.0	50.0	58.2	57.5	406-410 bp	1.0	77.0