Hotspot

rps16-trnQ-UUG

LSC · 6250-7811 · 1562 bp

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Pi 0.0354

MarkerSeek score 75.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1562
variable_sites: 78
pi: 0.0354
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1615-1635 bp	1.0	86.1
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1626-1646 bp	1.0	85.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GACGACCAACCACTACCAAT	45.0	50.0	58.1	57.5	1711-1731 bp	1.0	82.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GACGACCAACCACTACCAAT	50.0	50.0	58.2	57.5	1722-1742 bp	1.0	82.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1619-1639 bp	1.0	81.4