Hotspot

ycf2

Genome · 27711-34088 · 6378 bp

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Pi 0.017

MarkerSeek score 48.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6378
variable_sites: 108
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CTACGGAATCAGGGTGAAGC	TCGAATCCGTATCTATCCCACT	55.0	45.5	58.1	58.0	316 bp	1.0	65.3
ycf2_p2	GTGAAGCCGACGAAACTCTA	TCGAATCCGTATCTATCCCACT	50.0	45.5	57.7	58.0	303 bp	1.0	63.1
ycf2_p3	CTACGGAATCAGGGTGAAGC	TCGAATCCGTATCTATCCCAC	55.0	47.6	58.1	56.5	316 bp	1.0	61.7
ycf2_p4	CTACGGAATCAGGGTGAAGC	CGAATCCGTATCTATCCCACT	55.0	47.6	58.1	56.5	315 bp	1.0	61.6
ycf2_p5	TGAAGCCGACGAAACTCTAG	TCGAATCCGTATCTATCCCACT	50.0	45.5	57.4	58.0	302 bp	1.0	60.8