Hotspot

rpl36-infA

Genome · 75617-75668 · 52 bp

Back to Moerckia

Pi 0.135

MarkerSeek score 72.90

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: Genome
length_bp: 52
variable_sites: 7
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	GGCCGAAGAGTAAACCGATT	GGGTACAGTCACGGAATCAC	50.0	55.0	58.0	58.0	394-399 bp	1.0	81.6
rpl36-infA_p2	GGCCGAAGAGTAAACCGATT	GGTAACTCGTTGCGGAGAAT	50.0	50.0	58.0	58.0	516-521 bp	1.0	81.5
rpl36-infA_p3	TAGCTTTTTCTGTGGCGGTT	GGGTACAGTCACGGAATCAC	45.0	55.0	58.0	58.0	650-655 bp	1.0	81.4
rpl36-infA_p4	TAGCTTTTTCTGTGGCGGTT	GGTAACTCGTTGCGGAGAAT	45.0	50.0	58.0	58.0	772-777 bp	1.0	81.3
rpl36-infA_p5	GGCCGAAGAGTAAACCGATT	GAATTCTTTCGCGAGAGGGT	50.0	50.0	58.0	58.0	798 bp	0.5	59.7