Hotspot

trnS-GGA-rps4

Genome · 48102-48260 · 159 bp

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Pi 0.302

MarkerSeek score 72.60

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 159
variable_sites: 48
pi: 0.302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GACGAAGCTTTTGTGCTGTC	AGTTGCTCGAAATGCGTTTG	50.0	45.0	58.0	58.0	1243-1265 bp	1.0	83.7
trnS-GGA-rps4_p2	GACGAAGCTTTTGTGCTGTC	CAGTTGCTCGAAATGCGTTT	50.0	45.0	58.0	58.0	1244-1266 bp	1.0	83.7
trnS-GGA-rps4_p3	AGGGTACTCAACGGAAGGAA	AGTTGCTCGAAATGCGTTTG	50.0	45.0	58.0	58.0	1097 bp	0.5	59.5
trnS-GGA-rps4_p4	AGGGTACTCAACGGAAGGAA	CAGTTGCTCGAAATGCGTTT	50.0	45.0	58.0	58.0	1098 bp	0.5	59.5
trnS-GGA-rps4_p5	AGGGTACTCAACGGAAGGAA	TTCCTACGTCCCGTTATCGA	50.0	50.0	58.0	58.0	1358 bp	0.5	59.5