Hotspot

ycf3-trnS-GGA

Genome · 47767-48015 · 249 bp

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Pi 0.0161

MarkerSeek score 51.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: Genome
length_bp: 249
variable_sites: 4
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCGGCAACAATTGTAAAAGT	TCGAACCCTCGGTAAGCTAT	35.0	50.0	54.0	57.9	380 bp	1.0	48.5
ycf3-trnS-GGA_p2	TCGGCAACAATTGTAAAAGT	TTCGAACCCTCGGTAAGCTA	35.0	50.0	54.0	58.2	381 bp	1.0	47.7
ycf3-trnS-GGA_p3	TCGGCAACAATTGTAAAAGTT	TCGAACCCTCGGTAAGCTAT	33.3	50.0	54.9	57.9	380 bp	1.0	47.4
ycf3-trnS-GGA_p4	TCGGCAACAATTGTAAAAGT	GGATTCGAACCCTCGGTAAG	35.0	55.0	54.0	57.8	384 bp	1.0	47.2
ycf3-trnS-GGA_p5	TCGGCAACAATTGTAAAAGTT	TTCGAACCCTCGGTAAGCTA	33.3	50.0	54.9	58.2	381 bp	1.0	46.6