Hotspot

psaM-trnS-GCU

Genome · 19961-20347 · 387 bp

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Pi 0.0395

MarkerSeek score 70.30

Primer available yes

Label psaM-trnS-GCU

Feature Metadata

label_name: psaM-trnS-GCU
feature_type: igs
region: Genome
length_bp: 387
variable_sites: 15
pi: 0.0395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-trnS-GCU_p1	TTTCGTGGCAGGTATTCTGG	GAATGGCTCGTACAACGGAT	50.0	50.0	57.9	58.1	494-497 bp	1.0	82.2
psaM-trnS-GCU_p2	TTTCGTGGCAGGTATTCTGG	GCTCGTACAACGGATTAGCA	50.0	50.0	57.9	58.1	489-492 bp	1.0	82.1
psaM-trnS-GCU_p3	GAGTGTTTTCGTGGCAGGTA	GAATGGCTCGTACAACGGAT	50.0	50.0	58.1	58.1	500-503 bp	1.0	82.0
psaM-trnS-GCU_p4	GAGTGTTTTCGTGGCAGGTA	GCTCGTACAACGGATTAGCA	50.0	50.0	58.1	58.1	495-498 bp	1.0	81.9
psaM-trnS-GCU_p5	GCAGGTATTCTGGCCCTAAG	GAATGGCTCGTACAACGGAT	55.0	50.0	57.7	58.1	487-490 bp	1.0	81.0