Hotspot

rpoC2-rps2

Genome · 12609-12871 · 263 bp

Back to Moerckia

Pi 0.0539

MarkerSeek score 67.60

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: Genome
length_bp: 263
variable_sites: 11
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TCCGAAATAGCAGAAATATCT	ACCGAAATGAACACCTGCTT	33.3	45.0	52.1	58.0	301-358 bp	1.0	46.7
rpoC2-rps2_p2	TCCGAAATAGCAGAAATATCTT	ACCGAAATGAACACCTGCTT	31.8	45.0	53.1	58.0	301-358 bp	1.0	46.7
rpoC2-rps2_p3	TCCGAAATAGCAGAAATATCT	GGTGACCGAAATGAACACCT	33.3	50.0	52.1	58.1	305-362 bp	1.0	46.6
rpoC2-rps2_p4	TCCGAAATAGCAGAAATATCT	GCCATCTTGGGATTCCACTT	33.3	50.0	52.1	57.9	333-390 bp	1.0	46.4
rpoC2-rps2_p5	TCCGAAATAGCAGAAATATCT	ATAGGGTGCCATCTTGGGAT	33.3	50.0	52.1	58.2	340-397 bp	1.0	46.4