Hotspot

psbM-ycf66

Genome · 402-529 · 128 bp

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Pi 0.0547

MarkerSeek score 71.90

Primer available yes

Label psbM-ycf66

Feature Metadata

label_name: psbM-ycf66
feature_type: igs
region: Genome
length_bp: 128
variable_sites: 7
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-ycf66_p1	TCAATTCCAACCGCCTTTCT	TACTGGGGCCCAATTCCATA	45.0	50.0	57.7	58.1	216 bp	1.0	79.6
psbM-ycf66_p2	TCTCAATTCCAACCGCCTTT	TACTGGGGCCCAATTCCATA	45.0	50.0	57.7	58.1	218 bp	1.0	79.6
psbM-ycf66_p3	TCAATTCCAACCGCCTTTCT	CCGCACCTATTACGGTACTG	45.0	55.0	57.7	57.9	231 bp	1.0	79.1
psbM-ycf66_p4	TCAATTCCAACCGCCTTTCT	CGCACCTATTACGGTACTGG	45.0	55.0	57.7	57.9	230 bp	1.0	79.1
psbM-ycf66_p5	TCTCAATTCCAACCGCCTTT	CGCACCTATTACGGTACTGG	45.0	55.0	57.7	57.9	232 bp	1.0	79.1