Hotspot

rpl16

LSC · 80501-81801 · 1301 bp

Back to Moehringia

Pi 0.00461

MarkerSeek score 49.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1301
variable_sites: 6
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTCCTTTGGCTCTACGTTC	ACCTATTATTAATTCCACAAGGT	45.0	30.4	55.7	53.1	1384 bp	1.0	40.3
rpl16_p2	TTTCCTTTGGCTCTACGTTC	AACCTATTATTAATTCCACAAGGT	45.0	29.2	55.7	53.9	1385 bp	1.0	40.3
rpl16_p3	TTTCCTTTGGCTCTACGTTC	ACCTATTATTAATTCCACAAGGTT	45.0	29.2	55.7	53.9	1384 bp	1.0	40.3
rpl16_p4	TTTCCTTTGGCTCTACGTTC	AAACCTATTATTAATTCCACAAGGT	45.0	28.0	55.7	54.7	1386 bp	1.0	40.3
rpl16_p5	TTTCCTTTGGCTCTACGTTC	AACCTATTATTAATTCCACAAGGTT	45.0	28.0	55.7	54.7	1385 bp	1.0	40.3