Hotspot

ndhD

SSC · 114057-115556 · 1500 bp

Back to Moehringia

Pi 0.00133

MarkerSeek score 48.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 2
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTCACAAACGTCAAACACCC	TCTTTTCGGGTACGGGTTTT	50.0	45.0	57.5	57.4	1664 bp	1.0	71.3
ndhD_p2	CTCACAAACGTCAAACACCC	TTCTTTTCGGGTACGGGTTT	50.0	45.0	57.5	57.4	1665 bp	1.0	71.3
ndhD_p3	CTCACAAACGTCAAACACCC	TTTCTTTTCGGGTACGGGTT	50.0	45.0	57.5	57.4	1666 bp	1.0	71.3
ndhD_p4	CTCACAAACGTCAAACACCC	TCTTTTCGGGTACGGGTTTTT	50.0	42.9	57.5	58.1	1664 bp	1.0	67.8
ndhD_p5	CTCACAAACGTCAAACACCC	TTCTTTTCGGGTACGGGTTTT	50.0	42.9	57.5	58.1	1665 bp	1.0	67.8