Hotspot

atpE

LSC · 51589-51990 · 402 bp

Back to Moehringia

Pi 0.00995

MarkerSeek score 49.10

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 402
variable_sites: 4
pi: 0.00995
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	GTATTTACGCGCACTAACTGG	TGAACTTAGAAATGGAGAGCA	47.6	38.1	57.4	54.6	454 bp	1.0	40.7
atpE_p2	GTATTTACGCGCACTAACTGG	GCTATGAACTTAGAAATGGAGAGC	47.6	41.7	57.4	57.5	458 bp	1.0	40.7
atpE_p3	GTATTTACGCGCACTAACTGG	AGAAATGGAGAGCAAATTGAAGA	47.6	34.8	57.4	56.4	447 bp	1.0	40.7
atpE_p4	GTATTTACGCGCACTAACTGG	TGAACTTAGAAATGGAGAGCAA	47.6	36.4	57.4	55.4	454 bp	1.0	40.7
atpE_p5	GTATTTACGCGCACTAACTGG	ATGAACTTAGAAATGGAGAGCA	47.6	36.4	57.4	55.2	455 bp	1.0	40.7