Hotspot

ndhF-rpl32

SSC · 111122-111741 · 620 bp

Back to Moehringia

Pi 0.00806

MarkerSeek score 63.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 620
variable_sites: 5
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAGGGT	CTTTTAACGCCGCCCAATAC	50.0	50.0	57.1	57.8	763 bp	1.0	72.0
ndhF-rpl32_p2	ACGCCTACGATTTTCTGTTGA	CTTTTAACGCCGCCCAATAC	42.9	50.0	58.0	57.8	825 bp	1.0	70.7
ndhF-rpl32_p3	CTGGAAGTGGAATGAAGGGT	AAAAAGCTTTTAACGCCGCC	50.0	45.0	57.1	58.5	769 bp	1.0	69.4
ndhF-rpl32_p4	ACGCCTACGATTTTCTGTTGA	AAAAAGCTTTTAACGCCGCC	42.9	45.0	58.0	58.5	831 bp	1.0	68.2
ndhF-rpl32_p5	CTGGAAGTGGAATGAAGGGT	GAAAAAGCTTTTAACGCCGC	50.0	45.0	57.1	57.2	770 bp	1.0	66.8