Hotspot

rpl20-rps12

LSC · 68314-69105 · 792 bp

Back to Moehringia

Pi 0.00379

MarkerSeek score 60.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 3
pi: 0.00379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	908-909 bp	1.0	78.4
rpl20-rps12_p2	TGAATGAGCCCCTCGAAAAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	906-907 bp	1.0	78.4
rpl20-rps12_p3	GAATGAGCCCCTCGAAAAGT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	905-906 bp	1.0	78.4
rpl20-rps12_p4	GAGCCCCTCGAAAAGTTGAT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	901-902 bp	1.0	78.4
rpl20-rps12_p5	AGCCCCTCGAAAAGTTGATG	CGCCGAGGAACATGTACTAG	50.0	55.0	58.2	57.9	900-901 bp	1.0	77.9