Hotspot

rbcL-accD

LSC · 55701-56467 · 767 bp

Back to Moehringia

Pi 0.00261

MarkerSeek score 60.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 2
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCTCGCGAGGGTAATACTA	CCAAGGCTGTCCGTTGATTT	50.0	50.0	58.0	58.8	971 bp	1.0	73.9
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CCAAGGCTGTCCGTTGATTT	55.0	50.0	57.7	58.8	929 bp	1.0	71.8
rbcL-accD_p3	TGCTCGCGAGGGTAATACTA	CCAAGGCTGTCCGTTGATT	50.0	52.6	58.0	58.1	971 bp	1.0	71.5
rbcL-accD_p4	TGCTCGCGAGGGTAATACTA	TCAATAGGACCAAGGCTGTC	50.0	50.0	58.0	56.9	980 bp	1.0	71.3
rbcL-accD_p5	TGCTCGCGAGGGTAATACTA	AGGCTGTCCGTTGATTTACT	50.0	45.0	58.0	56.8	968 bp	1.0	70.7