Hotspot

psbM-trnD-GUC

LSC · 28279-28838 · 560 bp

Back to Moehringia

Pi 0.00714

MarkerSeek score 62.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 560
variable_sites: 4
pi: 0.00714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGCGCAGTAGC	TGTCTCCCCTAGTTCCTTGG	50.0	55.0	58.1	58.0	701-703 bp	1.0	79.5
psbM-trnD-GUC_p2	AATGAAGAGCGCAGTAGCAA	TGTCTCCCCTAGTTCCTTGG	45.0	55.0	57.9	58.0	699-701 bp	1.0	79.3
psbM-trnD-GUC_p3	AGAATGAAGAGCGCAGTAGC	CTCCCCTAGTTCCTTGGGAT	50.0	55.0	58.1	57.8	698-700 bp	1.0	78.3
psbM-trnD-GUC_p4	AATGAAGAGCGCAGTAGCAA	CTCCCCTAGTTCCTTGGGAT	45.0	55.0	57.9	57.8	696-698 bp	1.0	78.1
psbM-trnD-GUC_p5	ATGAAGAGCGCAGTAGCAAT	TGTCTCCCCTAGTTCCTTGG	45.0	55.0	57.7	58.0	698-700 bp	1.0	77.5