Hotspot

rpoB-trnC-GCA

LSC · 25898-26649 · 752 bp

Back to Moehringia

Pi 0.00266

MarkerSeek score 60.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 752
variable_sites: 2
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAAGCTCTTCCGATAAGCCC	CCGGATTTGAACTGGGGAAT	50.0	50.0	57.7	57.9	921-929 bp	1.0	76.4
rpoB-trnC-GCA_p2	AAAGCTCTTCCGATAAGCCC	AAAGGATTTGCAGTCCTCCG	50.0	50.0	57.7	58.2	901-909 bp	1.0	76.1
rpoB-trnC-GCA_p3	TCAGCTCATTTCCCTCTCGT	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.4	57.9	840-848 bp	1.0	75.5
rpoB-trnC-GCA_p4	TCAGCTCATTTCCCTCTCGT	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.4	58.2	820-828 bp	1.0	75.3
rpoB-trnC-GCA_p5	AAGCTCTTCCGATAAGCCCT	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.5	57.9	920-928 bp	1.0	75.0