Hotspot

trnS-GCU-trnG-UCC

LSC · 7575-8222 · 648 bp

Back to Moehringia

Pi 0.00772

MarkerSeek score 63.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 5
pi: 0.00772
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGAACACGGAAAGAGAGGGA	TAGTAATCAAACCGGGGGAC	50.0	50.0	58.0	56.6	822-823 bp	1.0	69.4
trnS-GCU-trnG-UCC_p2	CACGGAAAGAGAGGGATTCG	TAGTAATCAAACCGGGGGAC	55.0	50.0	58.1	56.6	818-819 bp	1.0	68.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TAGTAATCAAACCGGGGGAC	50.0	50.0	58.2	56.6	817-818 bp	1.0	68.2
trnS-GCU-trnG-UCC_p4	AGAACACGGAAAGAGAGGGA	ATAGTAATCAAACCGGGGGAC	50.0	47.6	58.0	57.1	823-824 bp	1.0	65.6
trnS-GCU-trnG-UCC_p5	CACGGAAAGAGAGGGATTCG	ATAGTAATCAAACCGGGGGAC	55.0	47.6	58.1	57.1	819-820 bp	1.0	65.1