Hotspot

ccsA-psaC

SSC · 116995-118868 · 1874 bp

Back to Mitella

Pi 0.0211

MarkerSeek score 64.50

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1874
variable_sites: 39
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TGTGGCTTCTATGGGGTTTC	CAGAAGACTGTGTCGGTTGT	50.0	50.0	57.5	57.8	2063-2084 bp	1.0	76.6
ccsA-psaC_p2	TGTGGCTTCTATGGGGTTTC	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.5	57.7	2043-2064 bp	1.0	75.8
ccsA-psaC_p3	TGTGGCTTCTATGGGGTTTC	GAGATGTGAATCCGCCTGTC	50.0	55.0	57.5	58.4	2041-2062 bp	1.0	75.0
ccsA-psaC_p4	TGTGGCTTCTATGGGGTTTC	GCCTGTCCAACGGATTTCTT	50.0	50.0	57.5	58.5	2028-2049 bp	1.0	74.7
ccsA-psaC_p5	GTGGCTTCTATGGGGTTTCT	CAGAAGACTGTGTCGGTTGT	50.0	50.0	57.2	57.8	2062-2083 bp	1.0	74.2