Hotspot

rpl32-trnL-UAG

SSC · 115395-115851 · 457 bp

Back to Mitella

Pi 0.0504

MarkerSeek score 72.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 457
variable_sites: 23
pi: 0.0504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGGAAATTCAAAAAGTTT	AGCGTGTCTACCGATTTCAC	30.0	50.0	52.0	58.0	528-548 bp	1.0	43.6
rpl32-trnL-UAG_p2	ACCGGAAATTCAAAAAGTTT	CAGCGTGTCTACCGATTTCA	30.0	50.0	52.0	58.0	529-549 bp	1.0	43.6
rpl32-trnL-UAG_p3	ACCGGAAATTCAAAAAGTTTT	AGCGTGTCTACCGATTTCAC	28.6	50.0	53.0	58.0	528-548 bp	1.0	43.6
rpl32-trnL-UAG_p4	ACCGGAAATTCAAAAAGTTTT	CAGCGTGTCTACCGATTTCA	28.6	50.0	53.0	58.0	529-549 bp	1.0	43.6
rpl32-trnL-UAG_p5	ACCGGAAATTCAAAAAGTTT	TTTAGAAGATCCCATGCCGC	30.0	50.0	52.0	58.0	593-613 bp	1.0	43.2