Hotspot

ndhF-rpl32

SSC · 114114-115235 · 1122 bp

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Pi 0.0209

MarkerSeek score 66.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1122
variable_sites: 20
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGACAGGAACAGGAAGTGGA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.9	58.0	1109-1271 bp	1.0	84.3
ndhF-rpl32_p2	CCGTCGGAAAAAGGAGAAGT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.8	58.0	1142-1304 bp	1.0	83.8
ndhF-rpl32_p3	AGACAGGAACAGGAAGTGGA	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.9	57.9	1117-1279 bp	1.0	83.3
ndhF-rpl32_p4	GGAACAGGAAGTGGAACGAA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.8	58.0	1104-1266 bp	1.0	83.2
ndhF-rpl32_p5	CAGGAAGTGGAACGAAAGGT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1100-1262 bp	1.0	83.1