Hotspot

petA-psbJ

LSC · 65160-65889 · 730 bp

Back to Mitella

Pi 0.014

MarkerSeek score 69.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 10
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.0	58.1	909-924 bp	1.0	73.4
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.0	57.8	910-925 bp	1.0	72.7
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	892-907 bp	1.0	72.5
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	906-921 bp	1.0	72.3
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	893-908 bp	1.0	71.8