Hotspot

atpB-rbcL

LSC · 56483-57249 · 767 bp

Back to Mitella

Pi 0.0175

MarkerSeek score 68.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 13
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGGAAAAGCTACATCCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	902-926 bp	1.0	74.6
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	901-925 bp	1.0	74.4
atpB-rbcL_p3	AATTTGAACGATACGCCCCA	CACCAGCTTTGAATCCAACAC	45.0	47.6	57.9	58.0	870-894 bp	1.0	74.3
atpB-rbcL_p4	AATTTGAACGATACGCCCCA	ACCAGCTTTGAATCCAACACT	45.0	42.9	57.9	58.1	869-893 bp	1.0	74.0
atpB-rbcL_p5	AAGCTACATCCAGTACCGGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	895-919 bp	1.0	73.9