Hotspot

trnY-GUA-trnE-UUC

LSC · 32362-32420 · 59 bp

Back to Mitella

Pi 0.0339

MarkerSeek score 65.30

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 2
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TAGCGAATTATTGGGCCGAG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	199 bp	1.0	77.4
trnY-GUA-trnE-UUC_p2	TAGCGAATTATTGGGCCGAG	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.8	57.7	220 bp	1.0	77.2
trnY-GUA-trnE-UUC_p3	CCAGCGTAGACATATTGCCA	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	168 bp	1.0	76.9
trnY-GUA-trnE-UUC_p4	ACCAGCGTAGACATATTGCC	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	169 bp	1.0	76.9
trnY-GUA-trnE-UUC_p5	CCAGCGTAGACATATTGCCA	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.8	57.7	189 bp	1.0	76.7