Hotspot

trnD-GUC-trnY-GUA

LSC · 31879-32277 · 399 bp

Back to Mitella

Pi 0.0258

MarkerSeek score 65.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 10
pi: 0.0258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	AGTATTTATTGGGTCCGCCG	TGGCAATATGTCTACGCTGG	50.0	50.0	57.8	57.8	525-531 bp	1.0	79.3
trnD-GUC-trnY-GUA_p2	AGTATTTATTGGGTCCGCCG	GGCAATATGTCTACGCTGGT	50.0	50.0	57.8	57.8	524-530 bp	1.0	79.3
trnD-GUC-trnY-GUA_p3	AGTATTTATTGGGTCCGCCG	GGGGACGGACTGTAAATTCG	50.0	55.0	57.8	58.4	546-552 bp	1.0	78.2
trnD-GUC-trnY-GUA_p4	AGTATTTATTGGGTCCGCCG	GGGACGGACTGTAAATTCGT	50.0	50.0	57.8	57.6	545-551 bp	1.0	78.1
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	463-469 bp	1.0	77.9