Hotspot

trnR-UCU-atpA

LSC · 10629-10825 · 197 bp

Back to Mitella

Pi 0.0383

MarkerSeek score 70.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 7
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ACAATCGGAATGAAAGGCGT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	58.2	58.1	360-364 bp	1.0	82.5
trnR-UCU-atpA_p2	ACAATCGGAATGAAAGGCGT	CATTCACCGAGGAAGCAGAA	45.0	50.0	58.2	57.9	368-372 bp	1.0	82.3
trnR-UCU-atpA_p3	ACAATCGGAATGAAAGGCGT	AAGACATTCACCGAGGAAGC	45.0	50.0	58.2	57.9	372-376 bp	1.0	82.3
trnR-UCU-atpA_p4	CAATCGGAATGAAAGGCGTC	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	57.5	58.1	359-363 bp	1.0	80.3
trnR-UCU-atpA_p5	ACAATCGGAATGAAAGGCGT	AGGAAGCAGAAGCCCTTTTG	45.0	50.0	58.2	58.4	359-363 bp	1.0	80.2