Hotspot

trnH-GUG-psbA

LSC · 77-403 · 327 bp

Back to Mitella

Pi 0.0536

MarkerSeek score 71.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 327
variable_sites: 17
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CCACTGCCTTATCCACTTGG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.2	57.9	394-402 bp	1.0	83.1
trnH-GUG-psbA_p2	CCACTGCCTTATCCACTTGG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.2	58.0	780-788 bp	1.0	80.6
trnH-GUG-psbA_p3	CCACTGCCTTATCCACTTGG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.2	58.0	779-787 bp	1.0	80.5
trnH-GUG-psbA_p4	CCACTGCCTTATCCACTTGG	CTGCGACTGCTGTTTTCTTG	55.0	50.0	58.2	58.0	954-962 bp	1.0	80.3
trnH-GUG-psbA_p5	CCACTGCCTTATCCACTTGG	ATATTCAGCTCCCGTTGCAG	55.0	50.0	58.2	58.0	974-982 bp	1.0	80.2