Hotspot

rpl16

LSC · 80289-81792 · 1504 bp

Back to Miscanthus

Pi 0.00182

MarkerSeek score 44.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1504
variable_sites: 7
pi: 0.00182
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.0	56.3	1659-1715 bp	1.0	42.6
rpl16_p2	TCCAGAAAGAAAAACCAGGGG	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.0	57.3	1651-1707 bp	1.0	42.4
rpl16_p3	CCAGAAAGAAAAACCAGGGGT	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.3	56.3	1658-1714 bp	1.0	40.4
rpl16_p4	CCAGAAAGAAAAACCAGGGGT	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.3	57.3	1650-1706 bp	1.0	40.4
rpl16_p5	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCAA	47.6	34.8	58.0	57.0	1659-1715 bp	1.0	40.4