Hotspot

ndhF-rpl32

SSC · 108169-109069 · 901 bp

Back to Miscanthus

Pi 0.00429

MarkerSeek score 48.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 901
variable_sites: 8
pi: 0.00429
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	989-1014 bp	1.0	44.4
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	961-986 bp	1.0	44.1
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	962-987 bp	1.0	44.1
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	963-988 bp	1.0	44.1
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	964-989 bp	1.0	44.1