Hotspot

rps3-rpl22

LSC · 82612-82672 · 61 bp

Back to Miscanthus

Pi 0.00984

MarkerSeek score 44.90

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 1
pi: 0.00984
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.9	57.7	229-230 bp	1.0	77.9
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.8	57.7	195-196 bp	1.0	76.6
rps3-rpl22_p3	CCAAGTCTGAAACCGAGTGG	AAATTCAGACCTCGAGCTCG	55.0	50.0	58.5	57.7	194-195 bp	1.0	74.6
rps3-rpl22_p4	GTGCGAACCAAAAGGAATGG	CGAGCTCGAGGACGTAGTTA	50.0	55.0	57.9	58.7	217-218 bp	1.0	74.5
rps3-rpl22_p5	CAAGTCTGAAACCGAGTGGA	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.5	57.7	193-194 bp	1.0	74.3