Hotspot

rpl20-rps12

LSC · 69371-70051 · 681 bp

Back to Miscanthus

Pi 0.00137

MarkerSeek score 44.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 2
pi: 0.00137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	761 bp	1.0	79.9
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	764 bp	1.0	79.5
rpl20-rps12_p3	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	776 bp	1.0	78.9
rpl20-rps12_p4	ACGCATTTTTGTTCGTCGTC	TGCAAGACAGCCAATACGAA	45.0	45.0	58.0	57.8	823 bp	1.0	78.7
rpl20-rps12_p5	GTTTGAGGCAAATGAACGCA	GTCGAGGAACATGTGCTAGG	45.0	55.0	57.9	58.1	779 bp	1.0	78.6