Hotspot

trnL-UAA-trnF-GAA

LSC · 50125-50502 · 378 bp

Back to Miscanthus

Pi 0.0222

MarkerSeek score 49.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 14
pi: 0.0222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	464-503 bp	1.0	78.6
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	CTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	481-520 bp	1.0	78.2
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	479-518 bp	1.0	78.2
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	TTTTTCTGTGCCAGGAACCA	50.0	45.0	57.5	57.8	486-525 bp	1.0	77.6
trnL-UAA-trnF-GAA_p5	AAGTCGTGAGGGTTCAAGTC	TTTTCTGTGCCAGGAACCAG	50.0	50.0	57.5	58.3	485-524 bp	1.0	76.4