Hotspot

psbM-petN

LSC · 18846-19650 · 805 bp

Back to Miscanthus

Pi 0.00108

MarkerSeek score 43.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 2
pi: 0.00108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCATTCTAGTCCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	47.6	50.0	57.3	58.2	953 bp	1.0	65.4
psbM-petN_p2	TCATTCTAGTCCCTACTGCCT	GTAGTATGGGGGAGGAGTGG	47.6	60.0	57.3	58.3	897 bp	1.0	64.4
psbM-petN_p3	TCATTCTAGTCCCTACTGCCT	TAGTATGGGGGAGGAGTGGA	47.6	55.0	57.3	58.4	896 bp	1.0	63.7
psbM-petN_p4	TCATTCTAGTCCCTACTGCCT	CGCTAGTAGTATGGGGGAGG	47.6	60.0	57.3	58.5	902 bp	1.0	63.0
psbM-petN_p5	TCATTCTAGTCCCTACTGCCTT	TAGTAAGTCTCGCTTGGGCT	45.5	50.0	58.0	58.2	953 bp	1.0	62.9