Hotspot

rps15

SSC · 124264-124536 · 273 bp

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Pi 0.00733

MarkerSeek score 41.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 2
pi: 0.00733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	TGATTTGCTTGCTCCTGAAA	45.0	40.0	58.1	55.8	1047-1096 bp	1.0	63.9
rps15_p2	CCACTGATAATGCCCACTCC	TGATTTGCTTGCTCCTGAAA	55.0	40.0	58.0	55.8	1604-1653 bp	1.0	63.6
rps15_p3	AAGGTAGATCGGCAGCTACT	TGATTTGCTTGCTCCTGAAA	50.0	40.0	57.9	55.8	1477-1526 bp	1.0	63.4
rps15_p4	CCATAAGGTAGATCGGCAGC	TGATTTGCTTGCTCCTGAAA	55.0	40.0	57.9	55.8	1481-1530 bp	1.0	63.3
rps15_p5	TAGATCGGCAGCTACTCCTC	TGATTTGCTTGCTCCTGAAA	55.0	40.0	58.1	55.8	1473-1522 bp	1.0	63.2