Hotspot

rps15-ycf1

SSC · 124537-124811 · 275 bp

Back to Mirabilis

Pi 0.0582

MarkerSeek score 68.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 275
variable_sites: 16
pi: 0.0582
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	TGCGGATTCCTACAGATTTCG	50.0	47.6	58.0	58.2	1036-1085 bp	1.0	72.3
rps15-ycf1_p2	CAGCAGTCGTTGACGTTTTC	CGGATTCCTACAGATTTCGCT	50.0	47.6	58.0	57.9	1034 bp	0.5	51.2
rps15-ycf1_p3	AACACCATGCATTGATGGGT	CGGATTCCTACAGATTTCGCT	45.0	47.6	58.1	57.9	1295 bp	0.5	50.7
rps15-ycf1_p4	TTGAAATTCAGCAGACCCCC	CGGATTCCTACAGATTTCGCT	50.0	47.6	58.1	57.9	917 bp	0.5	50.6
rps15-ycf1_p5	CAGACCCCCAGTTTTCTTCC	CGGATTCCTACAGATTTCGCT	55.0	47.6	58.1	57.9	906 bp	0.5	50.5