Hotspot

ndhI-ndhA

SSC · 120733-120811 · 79 bp

Back to Mirabilis

Pi 0.0506
MarkerSeek score 68.80
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
79
variable_sites
4
pi
0.0506
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 TCCAGTTATCATAGGGAACA ACAACTTCTTCCCAACTCCT 40.0 45.0 52.7 56.3 130-131 bp 1.0 42.5
ndhI-ndhA_p2 TCCAGTTATCATAGGGAACA ACAACTTCTTCCCAACTCCTT 40.0 42.9 52.7 57.0 130-131 bp 1.0 42.5
ndhI-ndhA_p3 TCCAGTTATCATAGGGAACAT ACAACTTCTTCCCAACTCCT 38.1 45.0 53.4 56.3 130-131 bp 1.0 42.5
ndhI-ndhA_p4 TCCAGTTATCATAGGGAACA AACAACTTCTTCCCAACTCCT 40.0 42.9 52.7 57.0 131-132 bp 1.0 42.4
ndhI-ndhA_p5 ATCCAGTTATCATAGGGAACA ACAACTTCTTCCCAACTCCT 38.1 45.0 53.4 56.3 131-132 bp 1.0 42.4