Hotspot

ccsA-ndhD

SSC · 116321-116559 · 239 bp

Back to Mirabilis

Pi 0.0711

MarkerSeek score 77.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 239
variable_sites: 17
pi: 0.0711
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAAATTCTGCAATTGTGGCT	ACCCAGATTTCGTTCTCTCAC	40.0	47.6	55.4	57.7	417 bp	1.0	52.0
ccsA-ndhD_p2	TGGATATGCTATTTTGGGGTCA	ACCCAGATTTCGTTCTCTCAC	40.9	47.6	57.2	57.7	376 bp	1.0	50.6
ccsA-ndhD_p3	CAAATTCTGCAATTGTGGCTT	ACCCAGATTTCGTTCTCTCAC	38.1	47.6	56.2	57.7	417 bp	1.0	50.3
ccsA-ndhD_p4	CAAATTCTGCAATTGTGGCT	CCCAGATTTCGTTCTCTCACT	40.0	47.6	55.4	57.4	416 bp	1.0	49.7
ccsA-ndhD_p5	CAAATTCTGCAATTGTGGCTT	CCCAGATTTCGTTCTCTCACT	38.1	47.6	56.2	57.4	416 bp	1.0	48.0