Hotspot

rpl22-rps19

LSC · 85503-85630 · 128 bp

Back to Mirabilis

Pi 0.0625

MarkerSeek score 70.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 8
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CACTTTTCTTTTCCGCCGTG	TTTCAGAGGACACGCAAAAA	50.0	40.0	58.2	56.1	195-202 bp	1.0	66.1
rpl22-rps19_p2	TCACTTTTCTTTTCCGCCGT	TTTCAGAGGACACGCAAAAA	45.0	40.0	58.3	56.1	196-203 bp	1.0	65.4
rpl22-rps19_p3	CACTTTTCTTTTCCGCCGTG	ATTTCAGAGGACACGCAAAA	50.0	40.0	58.2	55.9	196-203 bp	1.0	64.3
rpl22-rps19_p4	CACTTTTCTTTTCCGCCGTG	AATTTCAGAGGACACGCAAA	50.0	40.0	58.2	55.9	197-204 bp	1.0	64.2
rpl22-rps19_p5	TCACTTTTCTTTTCCGCCGT	ATTTCAGAGGACACGCAAAA	45.0	40.0	58.3	55.9	197-204 bp	1.0	63.6