Hotspot

rps8-rpl14

LSC · 82062-82304 · 243 bp

Back to Mirabilis

Pi 0.0391

MarkerSeek score 74.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 9
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCGGTGATGTTAGTAGATGCT	GAACTCGAGTTTTTGGTGCG	42.9	50.0	56.3	58.0	412-424 bp	1.0	62.2
rps8-rpl14_p2	TCAGCAATAGTGTCTTTACCCA	GAACTCGAGTTTTTGGTGCG	40.9	50.0	57.1	58.0	337-349 bp	1.0	61.8
rps8-rpl14_p3	TCGGTGATGTTAGTAGATGCT	ACTCGAGTTTTTGGTGCGAT	42.9	45.0	56.3	58.1	410-422 bp	1.0	61.4
rps8-rpl14_p4	TCAGCAATAGTGTCTTTACCCA	ACTCGAGTTTTTGGTGCGAT	40.9	45.0	57.1	58.1	335-347 bp	1.0	61.0
rps8-rpl14_p5	TTCGGTGATGTTAGTAGATGCT	GAACTCGAGTTTTTGGTGCG	40.9	50.0	57.0	58.0	413-425 bp	1.0	60.0