Hotspot

ycf4-cemA

LSC · 61706-62584 · 879 bp

Back to Mirabilis

Pi 0.0148

MarkerSeek score 67.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 13
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGAGATCCACCGGGGTAAAA	45.0	50.0	58.1	58.0	999 bp	1.0	80.0
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	AGAGATCCACCGGGGTAAAA	45.0	50.0	57.8	58.0	1015 bp	1.0	79.5
ycf4-cemA_p3	GCCATTCCCTTGACTCGTAC	AGAGATCCACCGGGGTAAAA	55.0	50.0	58.3	58.0	1065 bp	1.0	77.9
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	TGATAGAGAGATCCACCGGG	45.0	55.0	58.1	57.7	1005 bp	1.0	77.9
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	TGATAGAGAGATCCACCGGG	45.0	55.0	57.8	57.7	1021 bp	1.0	77.4