Hotspot

psbC-trnS-UGA

LSC · 35542-35768 · 227 bp

Back to Mirabilis

Pi 0.0354

MarkerSeek score 70.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 8
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCGATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.3	58.0	348-349 bp	1.0	79.8
psbC-trnS-UGA_p2	CCTGTTCTTTCGATGACCCC	GTTGGAGAGATGGCTGAGTG	55.0	55.0	58.3	58.0	352-353 bp	1.0	79.8
psbC-trnS-UGA_p3	CCTGTTCTTTCGATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.3	58.1	340-341 bp	1.0	79.6
psbC-trnS-UGA_p4	CCTGTTCTTTCGATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.3	58.1	341-342 bp	1.0	79.6
psbC-trnS-UGA_p5	CCTGTTCTTTCGATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.3	58.1	333-334 bp	1.0	79.6