Hotspot

psbI-trnS-GCU

LSC · 7812-8025 · 214 bp

Back to Mirabilis

Pi 0.0631

MarkerSeek score 73.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 13
pi: 0.0631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TTCGAATCCCTCTCTTTCCG	50.0	50.0	58.3	57.0	269-277 bp	1.0	76.3
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.3	56.9	272-280 bp	1.0	75.3
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	GGGTTCGAATCCCTCTCTTTC	50.0	52.4	58.3	58.1	272-280 bp	1.0	75.2
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	58.3	58.1	271-279 bp	1.0	75.2
psbI-trnS-GCU_p5	AATGATCCAGGGCGTAATCC	TTCGAATCCCTCTCTTTCCG	50.0	50.0	57.4	57.0	270-278 bp	1.0	73.9