Hotspot

ycf3

LSC · 46006-47977 · 1972 bp

Back to Mimusops

Pi 0.00203

MarkerSeek score 55.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1972
variable_sites: 4
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACTGATGGGGTCAACAAACA	TCGTTTTCCCACGAGAGGTA	45.0	50.0	57.3	58.4	2097-2099 bp	1.0	71.1
ycf3_p2	ACTGATGGGGTCAACAAACA	CGTTTTCCCACGAGAGGTAA	45.0	50.0	57.3	57.6	2096-2098 bp	1.0	70.6
ycf3_p3	ACTGATGGGGTCAACAAACAA	TCGTTTTCCCACGAGAGGTA	42.9	50.0	58.0	58.4	2097-2099 bp	1.0	68.9
ycf3_p4	TGACTGATGGGGTCAACAAA	TCGTTTTCCCACGAGAGGTA	45.0	50.0	57.0	58.4	2099-2101 bp	1.0	68.8
ycf3_p5	TTGACTGATGGGGTCAACAA	TCGTTTTCCCACGAGAGGTA	45.0	50.0	57.0	58.4	2100-2102 bp	1.0	68.8